mouse cxcl1 Search Results


kc standard  (R&D Systems)
94
R&D Systems kc standard
Kc Standard, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cxcl1 elisa kit  (R&D Systems)
95
R&D Systems cxcl1 elisa kit
Cxcl1 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa  (R&D Systems)
96
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cxcl1  (R&D Systems)
93
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Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for <t>CXCL1</t> (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm
Cxcl1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cxcl1 kc duoset elisa  (R&D Systems)
96
R&D Systems mouse cxcl1 kc duoset elisa
Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for <t>CXCL1</t> (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm
Mouse Cxcl1 Kc Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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mouse cxcl1 kc kit  (R&D Systems)
95
R&D Systems mouse cxcl1 kc kit
Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for <t>CXCL1</t> (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm
Mouse Cxcl1 Kc Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+cxcl1/Mouse+CXCL1%2FKC+Quantikine+ELISA+Kit/pmc07160935-226-7-10
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tumor bearing mice  (Proteintech)
93
Proteintech tumor bearing mice
Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for <t>CXCL1</t> (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm
Tumor Bearing Mice, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+cxcl1/Mouse+CXCL1+ELISA+Kit/pmc12996564-355-11-21
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anti kc antibody  (R&D Systems)
94
R&D Systems anti kc antibody
Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for <t>CXCL1</t> (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm
Anti Kc Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse cxcl1 kc  (R&D Systems)
93
R&D Systems mouse cxcl1 kc
Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for <t>CXCL1</t> (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm
Mouse Cxcl1 Kc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+cxcl1/Mouse+XL+CXCL1%2FKC%2C+Luminex%C2%AE+Performance+Assay/pmc10127687-196-7-19
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af 453na  (R&D Systems)
93
R&D Systems af 453na
Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for <t>CXCL1</t> (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm
Af 453na, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+cxcl1/Mouse+CXCL1%2FGRO+alpha%2FKC%2FCINC-1+Antibody/pm28739876-62-1-9
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Image Search Results


Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for CXCL1 (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm

Journal: Acta neuropathologica communications

Article Title: Polarizing receptor activation dissociates fibroblast growth factor 2 mediated inhibition of myelination from its neuroprotective potential.

doi: 10.1186/s40478-019-0864-6

Figure Lengend Snippet: Fig. 4 FGFR1 supports induction of pro-myelinating and neuroprotective factors. Mouse myelinating cultures were treated with 100 ng/ml FGF2 or F2 V2 at DIV18 for 3 days and protein levels in the cell culture supernatant were analyzed by ELISA for CXCL1 (a) and TIMP1 (b) or by Proteom Profiler (c, Mouse Cytokine Array Panel a). a, b FGF2 treatment results in a significant increase in protein levels for both CXCL1 and MMP3 (p < 0.05), whereas F2 V2 only significantly increased the levels of CXCL1. Data are presented as means + SEM from 6 independent experiments; * p < 0.05. c TIMP1 levels are increased in both FGF2 and F2 V2 treated cultures; data are presented as technical duplicates from 2 independent experiments, pixel densities are represented as arbitrary units. d-f A monolayer of neurosphere-derived mouse astrocytes was treated with 100 ng/ml recombinant FGF2 for 3 days and concentrations of CXCL1 (d), MMP3 (e) or TIMP1 (f) were determined by ELISA (d, e) or Proteom Profiler (f, Mouse Cytokine Array Panel a). d, e Data are presented as means ± SEM from 4 independent experiments; * p < 0.05; ** p < 0.01 (paired t-test), f Data are presented as technical duplicates from 2 independent experiments, arbitrary units represent pixel densities. g FGFR1 expression co- localizes with GFAP+ astrocytes in active multiple sclerosis. h, i Myelinating CNS cultures were treated in the absence or presence of 100 ng/ml FGF2 for 10 days from DIV 18 onwards and either directly fixed and stained for MOG / SMI31 after a total of 28 days (28 DIV) or after 38 days in vitro (38 DIV); shown are mean + SEM from at least 4 independent experiments and p-values for paired t-test; * p < 0.05. j Representative confocal immunofluorescence images (63x magnification, Maximum Intensity Projection) of 38 DIV culture demonstrating withdrawal of FGF2 (18 DIV – 28 DIV) is followed by rapid remyelination resulting in the formation of myelinated internodes that are indistinguishable from those in untreated cultures. Scale bars represent 10 μm

Article Snippet: Protein concentrations of CXCL1 and MMP3 in the supernatants were measured using mouse CXCL1 or MMP3 Quantikine ELISA kits (both R&D Systems) according to the manufacturer’s instructions.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Derivative Assay, Recombinant, Expressing, Staining, In Vitro, Immunofluorescence